FIRST REPORT ON PHYTOCONSTITUENTS, LC-ESI/MS PROFILE AND IN VITRO ANTIOXIDANT ACTIVITIES OF LATHYRUS LATIFOLIUS GROWING IN ALGERIA

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Keywords:

Lathyrus latifolius, antioxidant activity, hesperidin

Abstract

For the first time, this work is devoted to the phytochemical and biological study of a medicinal plant belonging to the Algerian flora Lathyrus latifolius. This research was conducted to assess the phytochemical composition of ethyle acetate ,butanolic and chloroform extracts using LCMS/MS, following by testing in vitro antioxidant ability using DPPH, ABTS·+, O2 − DMSO alkalin, Reducing power, β-Carotene-linoleic acid and CUPRAC assays. The liquid chromatography results showed that ethyle acetate extract have a high amount of Hesperidin (583.31g/ml) and Quercetin-3-D-xyloside(27.467g/ml), while the amounts present in the butanolic extract are respectively (3.360 g/ml) and (1.812mg/ml). Furthermore, butanol and ethyle acetate extracts had good antioxidant activity in all tests used. Indeed, the presence of phenolic compounds may contribute to their antioxidant activity.

Author Biographies

Selma Doudach, Université constantine1

Unit of Valorization of Natural Resources, Bioactive Molecules and Physicochemical andBiological Analyzes, Department of Chemistry, Faculty of Exact Sciences, Universityof Mentouri Brothers, P.B. 325 Route Ain El Bey, Constantine, Algeria

Nabila Slougui

Unit of Valorization of Natural Resources, Bioactive Molecules and Physicochemical andBiological Analyzes, Department of Chemistry, Faculty of Exact Sciences, Universityof Mentouri Brothers, P.B. 325 Route Ain El Bey, Constantine, Algeria

2 Ecole nationale polytechnique de Constantine. BP 75, A, Nouvelle ville RP, Constantine. Algérie

Khellaf Rebbas, Department of Natural and Life Sciences, Faculty of Science, University Mohamed Boudiaf of M’Sila,

Department of Natural and Life Sciences, Faculty of Science, University Mohamed Boudiaf of M’Sila, 28 000, Agro-Biotechnology and Nutrition Laboratory in Arid and Semi-Arid Zones /Natural Resources Management and Environment Team. Ibn Khaldoun University, Tiaret, Algeria

Lotfi Benmekhbi, université constantine3

 Laboratory of Materials Chemistry, University of Mentouri Brothers,P.B. 325 Route Ain El Bey, Constantine, Algeria

Chawki Bensouici, Biotechnology Research Center, Ali Mendjli Nouvelle Ville UV03,BPE73,Constantine,

Biotechnology Research Center, Ali Mendjli Nouvelle Ville UV03,BPE73,Constantine,

Mehmet Nuri Atalar, Faculty of Health Sciences, Department of Nutrition and Dietetics,I_gdır University, I_gdır, Turkey

Faculty of Health Sciences, Department of Nutrition and Dietetics,I_gdır University, I_gdır, Turkey

 

Salah Akkal, Unit of Valorization of Natural Resources, Bioactive Molecules and Physicochemical andBiological Analyzes, Department of Chemistry, Faculty of Exact Sciences, Universityof Mentouri Brothers, P.B. 325 Route Ain El Bey, Constantine, Algeria

Unit of Valorization of Natural Resources, Bioactive Molecules and Physicochemical andBiological Analyzes, Department of Chemistry, Faculty of Exact Sciences, Universityof Mentouri Brothers, P.B. 325 Route Ain El Bey, Constantine, Algeria

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Published

2022-12-14

How to Cite

Doudach, S., Slougui, N., Rebbas, K., Benmekhbi, L., Bensouici, C., Nuri Atalar, M., Akkal, S., & Bicha, S. (2022). FIRST REPORT ON PHYTOCONSTITUENTS, LC-ESI/MS PROFILE AND IN VITRO ANTIOXIDANT ACTIVITIES OF LATHYRUS LATIFOLIUS GROWING IN ALGERIA. International Journal of Natural and Engineering Sciences, 16(3), 128–136. Retrieved from https://ijnes.org/index.php/ijnes/article/view/694

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