FIRST REPORT ON PHYTOCONSTITUENTS, LC-ESI/MS PROFILE AND IN VITRO ANTIOXIDANT ACTIVITIES OF LATHYRUS LATIFOLIUS GROWING IN ALGERIA

Abstract

For the first time, this work is devoted to the phytochemical and biological study of a medicinal plant belonging to the Algerian flora Lathyrus latifolius. This research was conducted to assess the phytochemical composition of ethyle acetate ,butanolic and chloroform extracts using LCMS/MS, following by testing in vitro antioxidant ability using DPPH, ABTS·+, O2 − DMSO alkalin, Reducing power, β-Carotene-linoleic acid and CUPRAC assays. The liquid chromatography results showed that ethyle acetate extract have a high amount of Hesperidin (583.31g/ml) and Quercetin-3-D-xyloside(27.467g/ml), while the amounts present in the butanolic extract are respectively (3.360 g/ml) and (1.812mg/ml). Furthermore, butanol and ethyle acetate extracts had good antioxidant activity in all tests used. Indeed, the presence of phenolic compounds may contribute to their antioxidant activity.