Investigation of Protein and Polymer as a Carrier on Immune System
Abstract views: 53 / PDF downloads: 43Keywords:
Polyelectrolyte, biopolymer systems, immunogen, polymer-peptide conjugates, synthetic vaccine, foot-andmouth disease virus.Abstract
The immunogenic properties of water soluble BSA-Peptide and VP/AA-Peptide conjugates were investigated, and
specificity of antibodies produced was analyzed. This epitope of Foot-and Mouth Disease virus VP1 protein (135-161 amino
acid sequences) were used as a polypeptide antigen. Immunogenic activity of BSA-Peptide and VP/AA-Peptide conjugates
were investigated depending on the peptide amount in conjugates per mouse and carrier (synthetic polyelectrolyte or protein
molecule) structure. VP/AA-Peptide and BSA-Peptide conjugates revealed very high immunogenicity which are different in
regards to the specificity of the antibody produced. The BSA-Peptide conjugates were able to generate specific antibodies
both for 135-161 peptide epitope and BSA. In contrast to VP/AA-Peptide conjugates system was able to generate only 135-
161 peptide epitope antibodies.
