Characterization and Purification of Viral Peptides Synthesized with Microwave Assisted Solid Phase Method

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Authors

  • Zafer Ömer ÖZDEMİR
  • Murat TOPUZOĞULLARI
  • Erdem KARABULUT
  • Zeynep MUSTAFAEVA AKDESTE

Keywords:

LC-ESI-MS, RP-HPLC, solid phase peptide synthesis, synthetic vaccine

Abstract

Peptides and proteins play a central role in numerous biological and physiological processes in living organisms. This
study was conducted for characterization and purification of three different viral capsid peptide sequences. These viral
peptide sequences were synthesized with microwave assisted solid phase peptide synthesis in our research laboratory.
Synthesized viral peptide sequences are NP 55-69, melanoma 155-163, and VP1 135-161. Viral capsid peptides are part of
the viruses’ outer shell of genetic materials. Viruses are recognized by immune system via capsid peptides. Depending on
this property of capsid peptides, totally synthetic vaccine prototypes can be developed. LC-ESI-MS system was used to
characterize the sequences and peptides were purified with preparative HPLC system.

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Published

2019-07-15

How to Cite

ÖZDEMİR, Z. Ömer, TOPUZOĞULLARI, M., KARABULUT, E., & AKDESTE, Z. M. (2019). Characterization and Purification of Viral Peptides Synthesized with Microwave Assisted Solid Phase Method. International Journal of Natural and Engineering Sciences, 3(2), 45–48. Retrieved from https://ijnes.org/index.php/ijnes/article/view/470

Issue

Vol. 3 No. 2 (2009): 2009-2

Section

Articles