Molecular Genetic Analysis of Three Turkish Sheep Breeds by RAPDPCR Method

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Authors

  • F. Azize BUDAK YILDIRAN
  • Şükran ÇAKIR ARICA

Keywords:

genetic diversity, random amplified polymorphic DNA, sheep.

Abstract

In this study determined genetic polymorphisms within and among in three Turkish sheep breeds namely Sakız,
Karayaka and Bafra by the RAPD technique. These breeds were screened by 20 primers having arbitrary sequences.
DNA samples were extracted from blood. The samples were amplified by polimerase chain reaction (PCR). Amplified
products were resolved on 1.5% agarose gel and stained with ethidium bromide (EtBr). The RAPD data from 6 primers
were evaluated. Genetic distances were calculated which ranged from 0.040 to 0.470 between the breeds. The highest
heterozigocity was observed in Bafra breed. In addition, all individuals were tested in Bafra containing 1250 bp allel
which was assumed as a distinguishable character. This result can be suggested as a specific DNA marker for Bafra
breed.

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Published

2019-07-15

How to Cite

YILDIRAN, F. A. B., & ARICA, Şükran ÇAKIR. (2019). Molecular Genetic Analysis of Three Turkish Sheep Breeds by RAPDPCR Method. International Journal of Natural and Engineering Sciences, 3(3), 57–62. Retrieved from https://ijnes.org/index.php/ijnes/article/view/498

Issue

Vol. 3 No. 3 (2009): 2009-3

Section

Articles