Screening of F3 Segregation Population Lines Revealed by Ty-1 Markers Linked to Resistance Locus of Tomato Yellow Leaf Curl Disease (TYLCD) in Tomato (Lycopersicum. esculentum)

Abstract

Tomato yellow leaf curl virus (TYLCV) is one of the most devastating viral pathogens of cultivated tomatoes, causes severe losses in tomato production in tropical and subtropical regions. In this study, F3 plants originated from 11 F2 populations (individual numbers varied from 10 to 14 for each population, a total of 131 individuals) (Lycopersicum esculentum) were screened for resistance to Tomato yellow leaf curl virus (TYLCV) using Random Amplified Polymorphic DNA (RAPD) and Cleaved Amplified Polymorphic Sequence (CAPS) marker techniques. After DNA extraction from plants, CAPS primers were applied and screened for primer annealing of gene locus. Out of 131 plants,120 plants were detected containing gene locus. After that, the amplicons, obtained from PCR with CAPS primers (REXF1 and REX-R3), were digested with TaqI restriction endonuclease enzyme to identify whether the lines carrying resistance gene is homozygous or heterozygous. Hundred and five plants were found to be susceptible and 15 out of 131 were heterozygous for the resistance gene. Rest of the plants did not have primer annealing sites and no homozygous resistant lines were detected.