Effect of Different Culture Media on Cell Concentrations of Chlorella Pyrenoidosa under Photoautotrophic Conditions

Abstract

The influence of light intensity and media on the growth of the freshwater microalga Chlorella pyrenoidosa has been studied. Four media each
with 20, 35 and 55 μmol photons m-2s-1 light intensities resulting in twelve different combinations were studied. As light intensity increased from
20 μmol photons m-2s-1 to 55 μmol photons m-2s-1, there was a significant increase in cell concentration in all the media. Of the different growth
patterns observed, maximum cell concentration of 6.85 x 106
cells per ml was obtained in BG11 medium at 55 μmol photons m-2s-1 corresponding to
a specific growth rate (µ) of 0.327d-1 with a least doubling time (td
) of 50.86h. It was observed from this study that as light intensity increased from
20 μmol photons m-2s-1 to 55 μmol photons m-2s-1 media had no significant effect on the growth of alga.