The Effects of Ethylene Glycol on Factors Influencing Urolithiasis in Rat

Abstract

The aim of this study was to investigate in vitro the relative toxic effects of ethylene glycol (EG), a major industrial chemical, on biomarkers
of urolithiasis in rats. 60 adult male rats weighting 200-250 grams were randomly distributed into 10 groups of six each in two experiments. In
experiment 1, Four of the animal groups were each treated either with 0.2, 0.4, 0.8 or 1.6 percent EG in drinking water, respectively and one group
as control for a period of 4 weeks. Experiment 2, was exactly the same it, but for a period of 8 weeks. At the end of each of the treatment periods,
24-hour urine samples were collected from all respected animals using single metabolic cages. Urine samples were used to measure calcium,
oxalate, phosphorus, uric acid, protein, creatinine, citrate, magnesium and pH. Blood samples were used to measure calcium, phosphorus and
creatinine. Kidneys Sections were stained and pathologically investigated for stone formation. The results indicated EG or its metabolites caused
dose and duration of treatment dependent significant changes in urolithiasis factors. Thus considering health principles, the wide spread industrial
use of EG may not be as safe as it is thought to be.